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IPLUSO 19374

Cellular and Molecular Biology

Biomedical Laboratory Sciences
  • ApresentaçãoPresentation
    The course unit of Cellular and Molecular Biology introduces the knowledge concerning the structure and functioning of the cell.Fundamental knowledge concerning the storage, transmission and use of genetic information in eukaryotic and prokaryotic organisms is transmitted. The cell cycle and the control of gene expression are studied, as well as related themes such as cancer and apoptosis. The student should grasp the importance of molecular biology not only as a source of biotechnology processes, but also as a rapidly expanding toolkit with applications in multiple areas of health. In laboratory practice, the most commonly used techniques in biology will be performed.
  • ProgramaProgramme
    1.The eukaryotic and prokaryotic cell 2.Organelles and cell structures 3.Molecular organization of the cell 4.Cell Membrane 5.Organization of the eukaryotic and prokaryotic genome 6.Genetic Material 6.1. Replication and transcription of DNA 6.2. Gene expression and its regulation mechanisms 6.3. Mutation and repair mechanisms 6.4. Cell cycle and changes the cell cycle 7.Post-translational modifications, and protein degradation mechanisms 8.Molecular Biology Techniques 8.1. Extraction of DNA 8.2. Fundamental enzymes of molecular biology 8.3. Polymerase chain reaction (PCR) 8.4. molecular Cloning 8.5. DNA Sequencing 8.6. Electrophoresis and hybridization (Southern, Northern and Western blots) 8.7. Genetic typing 8.8. Probes and molecular markers 8.9. Techniques for manipulation of gene expression in eukaryotes 8.10.DNA microarrays 8.11.Detection of protein-protein interactions
  • ObjectivosObjectives
    1-Possess fundamental knowledge concerning the storage, transmission and use of genetic information in eukaryotic and prokaryotic organisms. 2-Possess general and specific notions of structure and function of different cell components. 3-To identify all the organelles and cellular structures in optical microscopy. 4-Having the ability to use the knowledge of animal and vegetal cell biology. 5-Cellular and Vegetal Biology in the critical analysis of information and practice of other disciplines. 6-Understand the molecular mechanisms of genetic and life processes in general. 7-Understand the importance of molecular biology as a source of biotechnology processes, providing a set of tools booming with applications in multiple healthcare disciplines. 8-Have basic laboratory skills in biology lab.
  • BibliografiaBibliography
    Azevedo, C. & Sunkel C. - Biologia Celular e Molecular 5th ed. Lisboa: Lidel editores. . 2012 ISBN: 9789727576920  Reece, J. B., et al.. - Campbell Biology. 9th ed.. Pearson Education, Inc.2011 .ISBN-13: 978-0321558237  Lodish, H., et al.  - Molecular Cell Biology. 7th ed.. W. H. Freeman and Co. 2012. ISBN-13: 978-1464183393  
  • MetodologiaMethodology
    In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
  • LínguaLanguage
    Português
  • TipoType
    Semestral
  • ECTS
    4
  • NaturezaNature
    Mandatory
  • EstágioInternship
    Não