IPLUSO 18936

Analytical Methods

• ApresentaçãoPresentation

  Provide students with skills on the main methods and techniques used in laboratory practice. Understand the theoretical basis of the methods and techniques used in laboratory lessons and consequent acquisition of the ability to choose suitable methods and techniques for each specific objectives. Understand the applicability of the techniques and methods introduced in the diagnosis and monitoring of various diseases.
• ProgramaProgramme

  Conteúdos programáticos: 1.            Segurança no laboratório 2.            Quimiometria. 2.1.         Análise e tratamento estatístico de resultados experimentais. 2.2.         Métodos de calibração. 3.            Métodos volumétricos de análise 3.1.         Volumetria ácido-base 3.2.         Volumetria redox 4.            Métodos espectrofotométricos 4.1.         Análise qualitativa e quantitativa em espetrofotometria uv/visível. 4.2.         Fluorescência e fosforescência. 4.3.         Métodos enzimáticos de análise. 5.            Técnicas de separação 5.1.         Centrifugação. Gradientes de densidade. Ultracentrifugação. 5.2.         Cromatografia. Técnicas cromatográficas e tipos de cromatografia. Cromatografia líquida, em gel, gasosa, de afinidade e permuta iónica. 5.3.         Eletroforese. Eletroforese em gel e acetato de celulose.
• ObjectivosObjectives

  Provide students with the following skills: Ability to perform laboratory tasks effectively and safely. Ability to properly interpret and treat the experimental results obtained in qualitative or quantitative methods, using statistical analysis. Ability to work independently and in a team in laboratory practice as well as in the communication of results and their critical evaluation Ability to perform laboratory tasks effectively and safely. Ability to interpret and process adequately the experimental results in qualitative or quantitative methods using statistical analysis. Ability to work in a laboratory either independently or as part of a team and to communicate and critically evaluate results.
• BibliografiaBibliography

  1. Harris, D.C. (2010). Quantitative Chemical Analysis. (8th ed.). W H Freeman. 2. Skoog, W., Holler and Crouch. (2000). Analytical Chemistry: An Introduction. (7th ed.). Harcourt College Publishers 3. Skoog, D. A., West, D. M., Holler, F. J. & Crouch, S. R. (2010). Fundamentals of Analytical Chemistry. (8th ed.). Thomson. 4. Christian, G. D. (2004). Analytical Chemistry. (6th ed.). John Wiley & Sons. 5. Pombeiro, A.J.L.O. (2003). Técnicas e operações unitárias em química laboratorial. (4th ed.). Fundação Caloute Gulbenkian.  
• MetodologiaMethodology

  In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, in order to ensure individual preparation and, subsequently, the discussion of procedures and results.
• LínguaLanguage

  Português
• TipoType

  Semestral
• ECTS

  4
• NaturezaNature

  Mandatory
• EstágioInternship

  Não