IPLUSO 19565

Histotechnology I

• ApresentaçãoPresentation

  In this curricular unit on histological techniques, the methodology for obtaining tissue sections thin enough to allow light or an electron beam to pass through, allowing the structure of cells / tissues and organelles, respectively, will be explained. Class planning is structured according to the different intrinsic stages of sample preparation, from reception, fixation, processing (dehydration, diaphanization and impregnation) and microtomy. Instruments for manipulating samples, techniques and protocols will be presented in detail. Although this technique is universally applicable, it does not work well in hard tissues such as bones, so the descaling technique will still be addressed.
• ProgramaProgramme

  Reception and handling of biological material derived from biopsies and surgery. Fixing the biological material. Fixing importance. The fasteners classification. Chemical fixation. Physical fixation. Fixing in special techniques. Fixing limitations. Decalcification. Descaling agents. Alternative methods of decalcification. Factors affecting the softening rate. Tissue processing. Principles of tissue processing. Processing steps: main reagents used. Alternative methods of tissue processing. Inclusion. Fragments guidance. Major alternative means of inclusion and inclusion means. Tissue Microarrays Microtomy. Types of microtomes. Cutting methodology rotary microtome. Extension cuts. Microtomy and conditions of the main problems during cutting.
• ObjectivosObjectives

  Know the histological techniques of preparing tissue for observation under the light microscope. Describe how the fasteners stop the postmortem changes; properties of an ideal fixer; Identify the advantages and disadvantages of different types of fasteners in view of its chemical properties; Describe the reason why the tissues need physical support for the histological section; Describe how to carry out the preparation of mineralized tissues; Describe the procedures for performing the inclusion in paraffin tissue blocks, main rules and resolution problems. Describe the procedures performed to perform histological sections, main rules, types of artifacts in histological sections and problem solving.
• BibliografiaBibliography

  Suvarna, S. K., Layton, C., & Bancroft, J. D. (Eds.). (2018). Bancroft's theory and practice of histological techniques (8th ed.). Elsevier. Kiernan, J. A. (2015). Histological and histochemical methods: Theory and practice (5th ed.). Scion Publishing. Luna, L. G. (1993). Histopathologic methods and color atlas of special stains and tissue artifacts. American Histolabs. Carson, F. L., & Hladik, C. (2021). Histotechnology: A Self-Instructional Text (5th ed.). ASCP Press. Sheehan, D. C., & Hrapchak, B. B. (1987). Theory and practice of histotechnology (2nd ed.). Battelle Press. Woods, A. E., & Ellis, R. C. (Eds.). (1994- ). Laboratory histopathology: A complete reference (2 vols.). Churchill Livingstone. Goldblum, J. R., Lamps, L. W., McKenney, J. K., & Myers, J. L. (Eds.). (2018). Rosai and Ackerman's surgical pathology (11th ed., 2 vols.). Elsevier.  
• MetodologiaMethodology

  Problem-based learning and laboratory case studies develop technical reasoning, analytical skills, and problem-solving related to the reception, fixation, decalcification, processing, embedding, and microtomy of tissues. The use of histological images, multimedia resources, simulations, technical demonstrations, and guided discussion of artefacts supports the understanding of procedures and the identification of errors. Practical activities reinforce autonomy, technical rigour, laboratory safety, and decision-making. The integration of digital platforms and interactive resources consolidates learning, stimulates critical thinking, and brings teaching closer to professional practice in Anatomic Pathology.
• LínguaLanguage

  Português
• TipoType

  Semestral
• ECTS

  5
• NaturezaNature

  Mandatory
• EstágioInternship

  Não