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IPLUSO 19565

Histotechnology I

Biomedical Laboratory Sciences
  • ApresentaçãoPresentation
    In this curricular unit on histological techniques, the methodology for obtaining tissue sections thin enough to allow light or an electron beam to pass through, allowing the structure of cells / tissues and organelles, respectively, will be explained. Class planning is structured according to the different intrinsic stages of sample preparation, from reception, fixation, processing (dehydration, diaphanization and impregnation) and microtomy. Instruments for manipulating samples, techniques and protocols will be presented in detail. Although this technique is universally applicable, it does not work well in hard tissues such as bones, so the descaling technique will still be addressed.
  • ProgramaProgramme
    Reception and handling of biological material derived from biopsies and surgery. Fixing the biological material. Fixing importance. The fasteners classification. Chemical fixation. Physical fixation. Fixing in special techniques. Fixing limitations. Decalcification. Descaling agents. Alternative methods of decalcification. Factors affecting the softening rate. Tissue processing. Principles of tissue processing. Processing steps: main reagents used. Alternative methods of tissue processing. Inclusion. Fragments guidance. Major alternative means of inclusion and inclusion means. Tissue Microarrays Microtomy. Types of microtomes. Cutting methodology rotary microtome. Extension cuts. Microtomy and conditions of the main problems during cutting.
  • ObjectivosObjectives
    Know the histological techniques of preparing tissue for observation under the light microscope. Describe how the fasteners stop the postmortem changes; properties of an ideal fixer; Identify the advantages and disadvantages of different types of fasteners in view of its chemical properties; Describe the reason why the tissues need physical support for the histological section; Describe how to carry out the preparation of mineralized tissues; Describe the procedures for performing the inclusion in paraffin tissue blocks, main rules and resolution problems. Describe the procedures performed to perform histological sections, main rules, types of artifacts in histological sections and problem solving.
  • BibliografiaBibliography
    Bancroft JD, Stevens A. (2007). Theory and Practice of Histological Techniques. (6th ed.). Edinburgh: Churchill Livingstone. Kiernan JA. (2015). Histological and Histochemical Methods. (5th ed.). United Kingdom: Scion Publishing Ltd. Luna L. (1993). Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. American Histolabs Pub Dept. Carson FL, Hladik C. (2014). Histotechnology: A Self-Instructional Text. (4th ed.). American Society for Clinical Pathology. Sheehan DC, Hrapchak BB. (1987). Theory and practice of Histotechnology. (2nd ed.). Batelle Memorial Institute. Woods AE, Roy CE. (1994). Laboratory Histopathology: A Complete Reference. 2 vols. Edinburgh: Churchill Livingstone.  Rosai J, Ackerman W. (2011). Surgical Pathology (2 vol). (10th ed.). Mosby.  
  • MetodologiaMethodology
    In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
  • LínguaLanguage
    Português
  • TipoType
    Semestral
  • ECTS
    5
  • NaturezaNature
    Mandatory
  • EstágioInternship
    Não