IPLUSO 19567

Histotechnology II

• ApresentaçãoPresentation

  In this curriculart unit, the methodology of staining histological sections and obtaining histological sections by freezing will be explained. Although the techniques addressed in the curricular unit of Histotechnology I (fixation, microtomy¿) have remained reassuringly constant, there have been great advances in immunological methodology, molecular and digital diagnostics. The progressive development of molecular techniques over the past 20 years, revolving around DNA and in situ hybridization, has led to the creation of new genetic testing and diagnostic opportunities for the laboratory. Digital pathology, in particular, is the new frontier, and quality control is critical to modern and relevant histotechnology.
• ProgramaProgramme

  1. Staining of Tissue Dyes: nomenclature Kinds of colors Hematoxylin-eosin staining Automated staining devices   2. Installation of histological sections Types of mounting means Manual assembly and automated assembly 3.Cryosectioning Methods of freezing Cutting methodology freezing microtome and cryostat Extemporaneous exams Muscle tissue freezing techniques for neuropathology 4. Photography and microphotography 5.Organization of the histopathology laboratory Organization of a routine clinical Quality control File blades and blocks Contamination at the Pathology Laboratory 6.Investigative techniques in histopathology DNA and RNA extraction from paraffin blocks 7.Preparation of body parts for display Methods fixation and preservation of the anatomical shape of the anatomical parts Presentation methods of body parts
• ObjectivosObjectives

  Know the techniques histological tissue preparation for observation under the light microscope and its applications in clinical pathology diagnosis and research. Perform the steps of the histopathological processing include in particular, blush and ride the histological sections. Describe the principles involved in the preparation of frozen sections. Compare different methods of freezing tissue. Identify the advantages of the frozen sections compared with other impregnation methods. Identify possible errors of histological processing and solve them properly. Recognize the importance of histopathological techniques in scientific studies. Understand histopathological techniques applied to research.
• BibliografiaBibliography

  Bancroft JD, Stevens A. (2007). Theory and Practice of Histological Techniques. (6 th ed.). Edinburgh: Churchill Livingstone. Kiernan JA. (2015). Histological and Histochemical Methods. (5 th ed.). United Kingdom: Scion Publishing Ltd. Luna L. (1993). Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. American Histolabs Pub Dept. Carson FL, Hladik C. (2014). Histotechnology: A Self-Instructional Text. (4 th ed.). American Society for Clinical Pathology. Sheehan DC, Hrapchak BB. (1987). Theory and practice of Histotechnology. (2 nd ed.). Batelle Memorial Institute. Woods AE, Roy CE. (1994). Laboratory Histopathology: A Complete Reference. 2 vols. Edinburgh: Churchill Livingstone. Rosai J, Ackerman W. (2011). Surgical Pathology (2 vol). (10 th ed.). Mosby.  
• MetodologiaMethodology

  In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
• LínguaLanguage

  Português
• TipoType

  Semestral
• ECTS

  5
• NaturezaNature

  Mandatory
• EstágioInternship

  Não