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IPLUSO 19535

Laboratory Techniques in Molecular Biology and Genetics

Bioanalysis and Control
  • ApresentaçãoPresentation
    No biological or biomedical training can dispense with understanding the role of molecular biology as a scientific support for multiple disciplines in the health area, namely in Bioanalysis. The almost exponential growth of Human Genetics at the end of the 20th century, culminating with the mapping of the human genome, allowed a better understanding of the entire clinical pathology. Laboratory classes are a complement to theoretical teaching and their main objective is to learn the most common techniques of molecular biology. At the end of the course, the student should be able to apply the concepts acquired in the interpretation and resolution of situations related to the practice of the profession.
  • ProgramaProgramme
    1. Techniques of Molecular Biology and Genetics 1.1. DNA extraction 1.2. Fundamental Enzymes of Biology molecular 1.3. Polymerase chain reaction (PCR) 1.4. Molecular cloning 1.5. DNA sequencing 1.6. Electrophoresis and hybridization (Southern, Northern and Western blots) 1.7. Genetic typing 1.8. Probes and Markers molecular 1.9. Gene expression manipulation techniques in eukaryotes 1.10. DNA microarrays 1.11. Detection of protein-protein interactions 2. Giemsa staining of metaphases 3. Application of concepts of population genetics
  • ObjectivosObjectives
    Understand the importance of molecular biology as a source of biotechnology processes, constituting a burgeoning toolkit with applications across multiple healthcare disciplines. ¿ Learn the specialized concepts of human genetics, the role of genes and the environment in normal development and in pathology. ¿ Hold specialized laboratory skills in Molecular Biology and Genetics laboratories. ¿Acquire attitudes and gestures necessary to perform laboratory tasks.
  • BibliografiaBibliography
    Goodwin W, Linacre A, Hadi. An Introduction to Forensic Genetics. 2 ed. John Wiley & Sons, 2010 nd ISBN: 978-0-470-71019-7 Butler JM; Advanced Topics in Forensic DNA Typing: Methodology, Academic Press, 2011 ISBN : 9780123745132
  • MetodologiaMethodology
    In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, in order to ensure individual preparation and, subsequently, the discussion of procedures and results.
  • LínguaLanguage
    Português
  • TipoType
    Semestral
  • ECTS
    6
  • NaturezaNature
    Mandatory
  • EstágioInternship
    Não