IPLUSO 18942

Clinical Biochemistry I

• ApresentaçãoPresentation

  Clinical Biochemistry will focus on the relationship between biochemical processes at the cellular level and the physiological processes that occur in the human body, focusing on examples of altered biochemical processes in human disease. In this sense, the student will have an integrated view of the various metabolic pathways and their regulation, with greater emphasis on clinical correlations, which describe biochemical states in disease states (with detail at the biochemical and genetic level), learning that biochemical parameters should be analyzed and monitored. This unit will have an important practical component where the student will use methodologies and analyze parameters used in a clinical laboratory in order to develop the critical spirit and the coherent form of presentation of results, which is fundamental in any professional area.
• ProgramaProgramme

  1 Introduction to Clinical Biochemistry 2 Sampling and Quality 3 Methods Used in Clinical Biochemistry 3.1 Laboratory Protein Evaluation: Protein Metabolism. Biosynthesis of non-essential amino acids. Diagnostic application of some proteins. Clinical Enzymology. Protein study methods .3.2 Laboratory evaluation of carbohydrates: Diabetes biochemistry. Methods of determination of carbohydrates .Functional tests; Glycated proteins. Pathogenesis of late diabetes complications. 3.3 Laboratory evaluation of lipids: Lipid metabolism; Biosynthesis of triglycerides; Biosynthesis of phospholipids. Lipoproteins: Methods of determination of lipids and lipoproteins; cholesterol, triglycerides, lipoproteins and ketone bodies 4 Chemical and cytological analysis of urine 5 Determination of proteins in urine 6 Determination of serum albumin. 7 Determination of glycated hemoglobin. 8 Serum determination of total cholesterol and triglycerides. 9 Determination of HDL cholesterol.
• ObjectivosObjectives

   - Global understanding and understanding of the fundamental biochemical mechanisms and their dysfunctions - Identify the type and how to obtain the biological product needed to determine each biochemical parameter.  -Know the analytical methods that can be applied in the quantitative and / or qualitative determination of a metabolite. -Learn current analytical techniques and platforms that are routinely used in clinical biochemistry and research - Know and apply the biochemical mechanisms necessary for the interpretation of clinical results. - Understand the implications of analytical and non-analytical errors. - Understand the principles of quality control
• BibliografiaBibliography

  Burtis, C., Ashwood, E.,Tietz. - Fundamentals of Clinical Chemistry, 6ª ed, Philadelphia,  W.B.Saunders Company, 2008 ISBN: 9781437719406 Kaplan, L.A. et al.- Clinical Chemistry: theory, analysis, correlation, 5th edition, St. Louis, C.V. Mosby Company, 2010 ISBN-13: 978-0323036580 McPherson, R and  Pincus, M.- Henry`s Clinical and Diagnosis Management by Laboratory Methods 22 th edition, W.S. Saunders Company 2011ISBN: 9780323247801 Anderson, S.; Cockane, S. - Clinical Chemistry Concepts and Applications, McGram- Hill, 2003 ISBN 13:  978-1-57766-514-4
• MetodologiaMethodology

  In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
• LínguaLanguage

  Português
• TipoType

  Semestral
• ECTS

  5
• NaturezaNature

  Mandatory
• EstágioInternship

  Não