IPLUSO 19606

Laboratory Techniques in Forensic Biology and Genetics

• ApresentaçãoPresentation

  The main objective of this course is to provide technical training in the laboratory scientific areas of forensic medicine, namely in Forensic Biology and Genetics. No biological or biomedical training can dispense with the understanding of the role of molecular biology as a scientific support for multiple health disciplines, particularly in legal medicine. The almost exponential growth of Human Genetics in the late twentieth century, culminating in the mapping of the human genome, allowed a better understanding of all clinical pathology. The laboratory classes are a complement to the theoretical teaching and have as main objective the learning of the most common techniques of molecular biology. At the end of the course, the student should be able to apply the concepts acquired in the interpretation and resolution of situations related to the exercise of the profession.
• ProgramaProgramme

  1. The application of genetic analysis in forensic medicine. 1.1 Principles of Molecular Biology 1.2 Proteins and Nucleic Acids1.3 Laws of Heredity 1.4 Mutation and Polymorphism1.5. Hereditary diseases1.6 Population genetics 2. Principles of applying DNA extraction techniques. 2.1 General extraction methodology 2.2 Methods of extracting different tissues. 2.3 The quality of extraction. 3. Principles of applying DNA amplification techniques. 3.1. DNA replication. 3.2. Enzymes used and molecular biology3.3. Conventional PCR3.4- Conventional PCR variants 4.Principles of applying PCR product detection techniques. 4.1 Molecular markers4.2 Clinical diagnosis (mutation detection - RFLPs, deletions) 4.3 Forensic diagnosis (amplification of different polymorphic regions) 4.4 Detection of viruses and pathogens 5.Choice and validation; quality control.
• ObjectivosObjectives

  Provide technical training in the scientific laboratory areas of legal medicine, namely Biology and Forensic Genetics. Promote scientific knowledge and laboratory and computer skills to understand the potential and limits of expert analysis in the field of Biology and Forensic Genetics. To transmit knowledge about the role of forensic genetics in the genetic identification of biological kinship, biological criminalistic and identification of cadaveric remains, as well as the laboratory course of the analyzes to be carried out and the genetic markers most used in the resolution of forensic cases.
• BibliografiaBibliography

  Goodwin W, Linacre A, Hadi. An Introduction to Forensic Genetics. 2nd ed. John Wiley & Sons, 2010 ISBN: 978-0-470-71019-7 Butler JM; Advanced Topics in Forensic DNA Typing: Methodology, Academic Press, 2011 ISBN: 9780123745132
• MetodologiaMethodology

  In practical classes, the student has prior access to the experimental protocols to be carried out in each class session, which allows for prior reading and preparation. These protocols are accompanied by a set of questions, to ensure individual preparation and, subsequently, the discussion of procedures and results.
• LínguaLanguage

  Português
• TipoType

  Semestral
• ECTS

  10
• NaturezaNature

  Mandatory
• EstágioInternship

  Não